Journal: Nature Communications

Article Title: Multi-level inhibition of coronavirus replication by chemical ER stress

doi: 10.1038/s41467-021-25551-1

Figure Lengend Snippet: a Scheme showing the expansion in a liquid−liquid interphase (LLI) followed by the differentiation at an air-liquid interphase (ALI) of normal human bronchial epithelial cells (NHBE). b Three-dimensional immunofluorescence analysis (z-stacks) of representative NHBE cells stained with antibodies specific for the indicated differentiation markers. Shown is one representative out of two biologically independent experiments. c NHBE cells were left untreated or infected with the indicated CoV (MOI = 3) and treated for up to three days with thapsigargin (0.1 or 1 µM). Supernatants were collected at five time points p.i. and virus titers determined by plaque assay. Data represent three biologically independent experiments using NHBE cells derived from two or three independent donors. Shown are means ± s.d. of technical duplicates. For HCoV-229E and MERS-CoV, cells from donor 1 were plated and differentiated a second time to generate an additional independent experiment (labeled donor 1.2). See Supplementary Fig. for cell viability experiments.

Article Snippet: Cryopreserved normal human bronchial epithelial (NHBE) cells were obtained from Lonza.

Techniques: Immunofluorescence, Staining, Infection, Plaque Assay, Derivative Assay, Labeling

Journal: Neoplasia (New York, N.Y.)

Article Title: Multimutated Herpes Simplex Virus G207 Is a Potent Inhibitor of Angiogenesis 1

doi:

Figure Lengend Snippet: Susceptability of human endothelial cells (HUVEC and HDMEC), fibroblasts (HFF), retinal glial cells (HRG), normal human bronchial epithelial cells (NHBE), and alveolar rhabdomyosarcoma (KFR) cell cultures to G207 and HSV-1 wild-type strain McIntyre. Cells were infected with G207 or McIntyre at an MOI of 0.1. Each data point (mean of triplicate wells ± SD) is the percentage of surviving cells compared with mock-infected cells in control wells or infectious viral titers at each time point, respectively. Some error bars for data points are smaller than the symbol size.

Article Snippet: Normal human bronchial epithelial cells (NHBE) were obtained from Clonetics (CellSystems, St. Katharinen, Germany) and cultured in BEGM medium according to the instructions of the producer.

Techniques: Infection, Control

Journal: International Journal of Clinical and Experimental Pathology

Article Title: Long non-coding RNA NKILA inhibits proliferation and migration of lung cancer via IL-11/STAT3 signaling

doi:

Figure Lengend Snippet: The expression of NKILA was higher in normal human bronchial epithelial cell line. For (A) and (B), the expression level of lncRNA-NKILA was analyzed by Q-PCR. The expression of NKILA was analyzed in NSCLC tissues (C), Kaplan-Meier analyses of the correlations between lncRNA-NKILA expression level and survival (D). Values are means ± SEM for n = 7-8. *P < 0.05, **P < 0.01, ***P < 0.001 vs. control.

Article Snippet: Cell culture A normal human bronchial epithelial cell line (16HBE) and NSCLC adenocarcinoma cell lines (A549, NCI-H1975) were purchased from the Biopike Biological company.

Techniques: Expressing, Control